RIPTIDE™ can generate a more comprehensive dataset of SNPs, and do so quicker and cheaper than microarray-based genotyping. Unlike microarrays, sequencing does not rely on established static content. The inherently unbiased and bottom-up approach of RipTide mandates equal probability of inclusion of all nucleotides in the genome and all members in a species. This eliminates any possibility of ascertainment bias that frequently arises in fixed-content approaches, such as microarray analyses. Moreover, RIPTIDE-enabled genotyping provides over 10 times the number of genetic markers at less than 10% the cost of a typical microarray.
Sanger Sequencing, while being familiar and partially cost-effective, generates huge datasets that require intensive data analysis. Unfortunately, these huge datasets are not always helpful because of their low sensitivity. Also, the cost-effectiveness decreases as your target number increases. RIPTIDE overcomes these challenges by allowing you to perform high-throughput library preparation.
16S RNA Sequencing
16S RNA sequencing is commonly used to identify microbiota within microbiomes because of its relatively short sequence. Its relative size results in it being cost-effective to traditionally. However, some microbes can have more than one 16S gene per cell, which can highly skew your results. Whole genome sequencing (WGS) neatly bypasses the copy number variant dilemma but is more expensive—until now! RipTide allows you to cost-effectively and efficiently perform WGS to identify microbiota within your microbiome of interest.